FIRST day in the pathogen lab.......

sux

the whole lab is damn smelly~~~~~~~~~~~

full of samonella smell............

zzzzzzzzzzzzzzzzzz..

so many things to rmb!! roarrssssssss......

For salmonella,

XLD--- Pink colonies with or without black centers. Many cultures of Salmonella may produce colonies with large, glossy black centers or may appear as almost completely black colonies.

BS agar-- black colonies; sometimes they have a metallic sheen. Surrounding medium is usually brown at first, but may turn black in time with increased incubation, producing the so-called halo effect.

HE agar--- Blue-green to blue colonies with or without black centers. Many cultures of Salmonella may produce colonies with large, glossy black centers or may appear as almost completely black colonies.



ok.. i have did some streaking in the TSI, LIA agar slant and stabbing of butt on the agar slant for the salmonella.. lols.. To test for vibrio cholerae, a loop of the surface pellicle of APW culture and transferred to the TCBS agar for streaking. I have done streaking using the suspected culture on the Arginine glucose slant (AGS) and stabbing the butt. And the salt test as well as the oxidase test for the Vibrio.

Sound chim? lols. the pathogen lab involved more on the streaking of plate, using of PCR, ABex, etc... lols.. I also helpout in the first lab.. cause chee meng was on mc tdy.. lols..

lots of stuff to do tdy.. haha.. tired.. but today is also the innovation day.. lols.. saw a lots of booth and games on going.. AND FOODS!!! haha.. Ms Ho asked all of us to go down to eat.. haha.. Then, they have decided to go down and bring those food for all of us to eat in the office.. lols.. So they took 3 plate of mee siam, cakes, candy floss, pop corn, etc... haha.. seems that micro lab de people all very hungry.. =P so ps.. haha.. I am realli getting fatter. Everyday eat,eat and eat. especially for days when we didnt have alot of samples.. lols

Tired !! ROARS TIME TO REST=P

tues 18.3.08

Mrs bay was on mc today!! Roars.. only me and cheemeng in the first lab.. nid to do all those stuff alone.. argghh stress... tio scolded by ms lye.. lols.. no choice.. I am worried about going to the pathogen lab on wed. as i am not realliy familarise with all the equipment and my theory realli sux. fk.. all those theory forgotten already!!! ROARSSS. realli nid to learn from the start... especially the incubating temp.



APW ---> Vibrio cholerae >>> 35oC

BPW ---> Salmonella >>> 35 / 41 degree celsius >> egg,

1/2 Fraser broth ----> Listeria 30 oC

shigella broth ----> shigella

campylobacter broth ---> campylobacter ----> 35oC

Rev broth ---> Escherchia coli ---> 35 oC



**List, campy and ecoli nid to label date and time in..



S.aueru: BP agar

E.coli : TBX agar

TPC: Standard plate count agar

VP: TXMS agar



mon 17.3.08

do spread plate, E coli coliform test, s. aureus, dilution. haha
Suppose to go pathogen lab today. But because serence have took leave... And she will return back to work on wednesday. So I will be going to pathogen lab on wed.. tired. alot of stuff to do.

tired!!

spread alot plates tdy... And did some coagualse testing today..

tml is audit in micro lab.. so no lab tml..

going to my friend de friend de department to help out and slack.. wahhaa..

sianz.. dunnoe what to eat for lunch tml.. lols..

tdy cheemeng help me dabao my lunch in e morning.. then bring there to eat

their canteen food realli suck..

sianz..

i am going to the pathogen lab next week... stress..

haha.. today they have order catering buffet for lunch using the welfare fund...

lots of stuff to eat..

at least better than e canteen food there =P

haha.. our canteen only have 1 stall which sell 'cai fan'.. so pathetic right? lols..

all those lab works is as usual. but get to learn more..

I have done streaking of agar plate the coagualses test for staphylococcus aueus...

and do some gram staining today..

very tired today.....

working tdy again.. lols..

get to cut the food sample and weight out today.. lols

and learn how to pipette and do spiral plate using the machine.. lols

there was some filming in the lab today and wasted some of the time

lol.. damn tired..

ROARS

I MISS MY HOLIDAYS

First day of attachment.. I am super afraid.. scared and STRESS
ok.. early in the morning, i took cab ..
well, most taxi driver dont know how to go perahu road..
so 2 cab rejected to drive me there.. =(
wait and wait.. and it is almost 8am already... so the next cab.. I just told him i was in rush of time.. ..and i asked him to try to drop me off somewhere around lim chu kang area. lols.. he asked me 'where is perahu road??' lols.. and give me his street directory.. to find map for him.. think he turn circles and circles.. tt why my cab fare today is super expensive.. then when i reach.. i was being brief and informed tt i will be attached to micro lab for 10 march until 25 april.. and then from 28 april i will be in the pesticides resicides department.. haha.. I think tt it is a good opportunity for me to be able to try out both of the department =) at least i dun have to do repeated stuff daily.. lols..
ok.. there was also a other attachment students from NUS, NP and SP tt have been working for some times in the micro lab.. at least one week before me or so.. lols..
ok basically my job scope is do microbiological food testing..
At first, i was told to count colonies.. zzz.. counting colonies is realli boring.. lols..
especially when using machine.. labelling of plate is also boring.. lols..
i get to spread plates, prepare camphylobacter media.. haha.. now they i know how to prepare media.. nid supplements.. and all those other ingredient..
and i also get to test all those food samples.. like fishball, meat, tuna, etc...
it's quite fun there ba.. firstly we did the dilution.. lols..
learning how to use machines and other equipment to pour buffer phosphate in to the bags.. to weight the bags.. to stomachakle the bag.. then using pipette to place in various agar.. and do spread plate.. lols.. but i am quite stress.. cause all those other attachment student did very well... lols.. of cuz.. we completed all our plating at 4 plus.. and start to sit there to chat while waiting for time to pass.. lols.. cause e bus will only arrive at 6.. lols.. ok.. i reached home around 7plus.. tired.. totally no mood to quarrel with somebodys

so fast .. it's friday..

coming to the end of my 1 week holiday.. lols..

I guess i have spent most of the time sleeping, mapling and going out..

simply lazy and slacking during this 1week

the more i slept, the more tired i am

At last..

The exam are finally over.. XD

All the exam papers are totally a nightmare for me...

Memorise.. memorise.. memorise.... ROARS!!!!!!

I know how badly i have done..

But there is no use.... what done cannot be undone..

I cant reverse back the time..

Anyway, there is still 1 week of holidays to enjoy..

followed by, the start of my attachment on 10 march

The time is always so short and yet, there is so many things that i wanted to do~~